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KMID : 0363320180390061244
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Journal of Korean Oriental Internal Medicine
2018 Volume.39 No. 6 p.1244 ~ p.1255
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Modulatory Effects of Herbal Medicines Extracts on Cytokine Release in Immune Response of RAW 264.7 and TK-1
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Bae Su-Kyoung
Cho Se-Hee
Ahn Tae-Kyu
Kim Jee-In
Kim Bong-Hyun
Lim Jae-Hwan
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Abstract
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Objectives: The purpose of this study is to determine the stimulatory effects of herbal medicines extracts on cytokines release of immune response in immune cells, RAW 264.7 and TK-1 cell.
Methods: In a total of 18 extracts, 9 water extracts and 9 ethanol extracts, of herbal medicines, the quantities of polyphenolic compounds were measured and anti-oxidation activities were determined by colorimetric assay. The herbal medicine extracts were treated on RAW 264.7 and TK-1, respectively, and then the releasing changes of tumor necrosis factor-α (TNF-α), interleukin-6, and interleukin-10 from both immune cells were determined by the enzyme-linked immunosorbent assay.
Results: The polyphenol contents were measured to be 1.56~0.64 mg/g of solids in the two types of extracts with 9 kinds of herbal medicines, while antioxidant activities were found to be 95.62~31.46% as compared with ascorbic acid control. In RAW 264.7 cells treated with herbal medicines extracts, the secretion of TNF-α increased to 1.31~1.18 fold, and the amounts of IL-6 were 68.4~97.9% compared with the control group treated with LPS alone. In particular, the secretion amount of anti-inflammatory cytokine IL-10 was suppressed by treatment using herbal medicine extracts. In the case of TK-1 cells, TNF-α secretion was suppressed according to the concentrations of herbal extract. The released amounts of IL-10 were shown at 10~40 pg/ml, and increased in a dose-dependent manner.
Conclusions: Herbal medicines extracts act on macrophages inducing the secretion of inflammatory cytokine, thereby enhancing the activity of innate immunity. When acting on T cells involved in adaptive immunity, the secretion of anti-inflammatory cytokine is increased to induce the inhibition of the innate immune response.
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KEYWORD
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herbal medicines, cytokine secretion, RAW 264.7 cell, TK-1 cell
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